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hello workshop participants welcome to the synthetic biology implementation tuck that I'm going to provide for you today happy to be here today I'm an assistant professor of biotechnology at the University of New Hampshire in Manchester New Hampshire my name is Kristen Johnson and I just want to let you know ahead of time that several of the documents that I'm going to talk about in this little presentation I provided to Nathan that I think he's going to put up on a folder for you today or at some point for you to be able to access and I'll refer to those at the end so the goals of my synthetic bio cure are to encourage critical thinking to build a laboratory community to teach communication in general but also science communication in particular to foster cooperation and collaboration and I'm going to go over each one of these and talk about some examples of how I do this with the symbiotic your'e and just as a little bit of background about me I'm in my third year teaching and I started teaching this in biocare two years ago so this coming year will be my third year teaching it and I really enjoy teaching it certainly come with bumps and a little bit of bruises along the way but it's been really really exciting to teach I also teach a cell culture cure as well as this one so in terms of course mechanics the synthetic biology laboratory kind of sits in a lecture course as well called molecular biology and biotechnology this is an advanced lecture lab elective course for our biology and biotechnology majors it's a has a prerequisite of genetics in our curriculum the lecture meets for one and a half hours two times per week and a lab meets for two hours two times per week usually Tuesdays and Thursday so it's separated by one day our students have access to the lab where this course is held nearly at all times when the lab the lab itself or the course is not in session so that's a really nice feature of the way we have this set up the class is capped at 16 students and all the times that I've run it I've have had 16 students in the class and as I mentioned it's for our biotech and biology majors and when I front it we've had nearly all juniors in seniors I think one time I've had a sophomore that's been able to take the class I've been fortunate to have undergrads as TAS both times seniors who have taken the course the year prior and they've been an excellent help and resource for this particular course so just as an introduction to how I run this lab you may be kind of partway through learning about kind of the mechanics of how this in bio lab works so some of this may be stuff you've already learned about some of it you may be learning about but the way that I run kind of introducing the material is very similar to the way that Ruth and Nathan I think do it for their horses so in week one in the lab I do an introduction basically overview what synthetic biology is we focus on one of the projects that's come out of the eye gem teams called vasila Phila which is essentially a concrete material that's been made out of bacterium and the students read some scientific articles about these look at the iGEM website where a student team has actually created these bacteria so just learn a little bit about synthetic bio we then have the students look at former I gem projects in small groups to try to understand a little bit about those IgM web sites in addition you can see both at the bottom of week one and the bottom of week two that there are these math packets and I'm happy to provide these for you guys to look at I have my students because they come in perhaps with different math skill sets these are pretty basic but they're essentially science basic chemistry math for the students to be able to do deletions and things like that and I have them work on those in the first couple of weeks outside of lab as homeworks in week two we get down and dirty with techniques to make sure that again all the students are on the same page learning basic pipetting skills so that they know how to use the pipette pumps they know how to use the micropipette which ones to use at different time so that when we actually get into the wet lab they understand how to use those and we do that through a serial dilution lab where they practice what they've done actually on paper in the math packet doing a protein standard curve and then utilize the microplate reader that they might actually use later when they're carrying out their sin bio project so they learn how to use some of the tools that they'll actually use in this in bio lab in so I did this this past year in prior years I've actually had them do a DNA spec lab learning how to use our spec to look at and calculate DNA concentration so either or seem to be great ways for them to learn how to do micro pipe cutting and use the tools for the labs in week 3 they start to learn about kind of the breakdown of the project exactly how they can work on the project so what our bio bricks they look at a 3/8 assembly overview and then they do that really cool restriction enzyme and ligase activity with the paper the scissors those restriction enzymes the ligase the tape in small groups and then start their team brainstorming for what their projects might actually look like then we spend week 4 to 12 in our semester doing team project work so some of the ways that I work on encouraging critical thinking in this particular course is again starting with that paper modeling of 3a assembly I really let the students struggle a bunch with this I let them each individually work on that paper modeling using those scissors using that tape to put things together and my TA does a wonderful job of walking around encouraging them to try try again and that really starts off the semester quite well with having them really kind of problem-solve both individually and then in small teams how does this all work together and they really get the idea of what this cure is all about how they're really going to have to figure things out on their own and that their answers are not going to be provided to them um then it comes to the experimental design they're working in teams they have to figure out what their device is going to look like how are they going to design this device what are the parts that they need to put together and then they come up with a proposal that they present to the rest of the class when they come up with that proposal they also need to propose how they're going to test and analyze that device so they have to really think about and design a device that has some method that they're able to analyze it and they have to put that in there to vise proposal and then of course as they go through the experiment throughout the semester there might be some process refinement we certainly had some troubles this past time where we went through our process or went through our semester and some of the device parts that students want wanted just didn't come out of our application processor or rather we tried to transform them into the e.coli and the transformation never worked for example so we actually never were able to get the part um so it never came out of the kit plate so they had to go and look for a different part a different device part and refine their process one of the other things that you'll probably hear about in the workshop as a suggestion is prior to them ever doing as I'm appear miniprep protocol make sure that when they go through that protocol step by step they are indicating on the protocol where is their plasmid DNA so they're writing down okay in this stuff that's in the super named in this stuff is in the poet that way they really understand even though it's you know all of those solutions are proprietary they really understand what's going on where their plasmid is and what's going on at each step that has them really think about what is happening in each step and when things go wrong and they certainly well as I mentioned just one thing earlier with us not being able to get certain devices that students wanted really encourage the students to stop think work in their teams if you are working with teens and really write things down step by step work together and work with you if you have a TA work with the TA to talk through things slowly and critically think through things we have found this to be really really helpful with the students on the right-hand side of this slide I happen to have a really nice diagram that one of my TAS this past year made in bio render to kind of help the students we laminated these to help the students think about the stepwise process that they go through each time they're cloning one of the device the device BioBricks into one of their plasmids or two of the biobrick parts into a plasmid and it helped them kind of isolate what stuff they were at sometimes they would take markers and circle the stuff that they were at and leave that laminated sheet kind of in their drawer so that when they came back in the next day they knew where they were at as I mentioned building web community was really really important we talked a lot about how we were functioning almost like a research lab each of us had our own projects in the lab you can see here just an example of one of our groups here working and each of us had our own team but we also had a really important role to play as an entire group within the lab in order to really build this lab community and make sure that teams are going to be really solid and work together really well the first three weeks of activities that I had I made sure that the students in the lab worked in different lab groups in order to get to know each other so that when they finally chose the teams they were going to work with for the eight week long synthetic biology project they made sure that those were people they were going to be able to work well with for that eight week long project so I essentially force students to move about the room for the first three weeks and work with different students in different lab groups and that seemed to work really well despite the fact that I was forcing it to make sure that they got to know one another quite well in addition I make students choose team names they identify who they're going to work with for their synthetic biology semester-long project this really gives them a sense of identity everything that they're doing from there on out there boxes for their plasmids their ice chests all of those sorts of things have their team names often it's with initials but they have teams that really identify essentially the the core concept behind their project and they identify they have project ownership they'll walk around talking to others about and mentioning to others about what their team name is and when they present their project in front of us they present it initially with their team name that ends up on their poster as well and I find a found over the last couple of years that that idea of a team name really does ultimately give a lot of project ownership to the students in the student groups in addition as I think Ruth and Nathan do we color code everything so you might be able to see a little bit here on this little styrofoam ice chest there's yellow this was the yellow team in the back here you can see some pink of this picture this was the pink team and there's a little bit of pink on the edge of this bench so everything that corresponding to this team here was yellow and we had six different benches in the lab and each had a particular fluorescence colored duct tape and everything that went with it and this really helps with organization and I really appreciated the suggestion coming out of this is that if I allowed to do this color coding well it took a little bit of time the organization that comes out of that helped and it really helps build the lab communities so students know where everything goes how everything goes and how to organize well organize a lab um in addition we really work on building lab community through a program that I've set up with the help of finding some resources online called lab citizenship and research habits of mind and this is an evaluation that I use to set expectations and this is one of those resources that I'm happy to share with you as I said I found some resources online to help do this but this sets expectations for how do you act to be a good lab citizen what does it mean to take care of your own space what does it mean to dispose of materials properly in terms of bio hazards in terms of other things what does it mean to clean up after yourself in the common lab areas now we share this lab with other labs other advanced biology courses making sure that all the equipment is taken care of making sure that after you use the weigh scale after you use the centrifuge that you're putting it back in working order so the next group even within our own class the next group is able to use it and everything is taken care of quite well and even going above and beyond if you see something that's not in its right working order that you take care of it yourself the research habits of mine go beyond that those are kind of some of the critical thinking aspects that I mentioned before and again I have an evaluation form for that I use that at the midterm students do a self-evaluation to let me know how they think they're doing I also give them an evaluation at that time as well and then there's an end of the semester evaluation I found that to be really useful I just started with that this year I thought it should be really useful for students to really reflect on how are they doing in terms of helping build that lab community and then how are they doing in terms of their their research excuse me their research aspects their ability to critically think their ability to ask questions and participate in different aspects of the lab like lab meetings like kind of working as a team as you can see this particular group is doing here how are they doing collaborating with their team members in terms of science communication and communication in general lab notebooks are extremely important for our lab in fact because we're a biotechnology program I have my students keep GNP or GLP level lab notebooks so there's a whole series of requirements associated with that that kind of ran the specificity of exactly how precise they are keeping those lab notebooks what kind of documentation is required in addition to that in those specs one of the most important things that I stress in that lab notebook is at the end of each day they should end their lab entry with the next steps note so that they come in whenever it is they come in next they or their teammates that come in next know what they are planning to do you the next time they come in sometimes someone might be absent someone might be sick and anyone should be able to walk in look at the notebook and understand what it is they need to do the next day and that has been really helpful for the teams to make sure that they progress in a positive way there's no missing elements there's no missteps I mentioned lab meeting presentations just like the concept of any research lab and I as I mentioned I encouraged my students to understand how our research lab runs the concept of a lab meeting is something that we've introduced just this past year I explained to students how lab meetings are very important for research labs that run with students I had postdocs and those sorts of things and so what we do is we set up several dates that the students can sign up for and during those dates a team will get up in front of the rest of us and give us an update on how their project is going they present to us their original design and then they update us on how their cloning is going for their devices how far they've gotten any sort of roadblocks they've met and then they get feedback from the other teams maybe one of the other teams has met an equivalent roadblock maybe they're cloning the same promoter and they're having the same problems or different problems maybe they can offer some other support or suggestions and we've had some really good success this past year with these lab meeting presentations which leads to some collaborations which I'll mention in the next slide in terms of other communication I have my students write individual lab reports so even though they work as teams typically teams of three each individual student writes a separate lab report which is greeted at the end of the semester and then they do poster presentations so the team altogether the team of three puts together a poster you can see some examples of student posters that came about from this year they're presented our undergrad research symposium at the end of the semester and again they work together in their team of three to put together the poster talk about their materials and methods and present any data that they have at that point so as I was mentioning in the last slide I like to make sure we're fostering also cooperation and collaboration obviously there's much cooperation that's required within the teams in order to make sure that their success success has been seen most with the teams that are cooperating collaborating communicating best certainly communicating with those lab notebooks and cooperating well those teams that are not communicating and not cooperating have despite the fact that they might be you know earning good grades otherwise they have not been successful with a synthetic bio home project but what we've seen very interestingly this particular this last year in particular is those lab meetings have brought out a new element to this project and that's collaboration between teams as I mentioned one team may be presenting something in a lab meeting where they've seen something happening with a particular promoter they've had either some success or some troublesome failure and another team recognizes that they might be cloning the same promoter or the same other portion of the device and after the meetings over I noticed the two teams talking in the corner of the classroom about oh can I use this portion of your device or here I'll offer you this portion of my device I stopped them this year and I said you know what you guys just engaged in a research collaboration why don't you talk about how this goes talk about you know what you're giving what you're getting how this collaboration goes agree upon it make sure that later on when you present your poster when you present your research paper you acknowledge this collaboration you acknowledge where you got this portion of your device and I thought there was a fantastic outcome of this research kind of environment that I was trying to put forth this year so who's really excited about that and ended up talking about it with the entire group and the entire class later on one of the other things that we talked about because we're a biotech program as well as we talk about networking and we talked about how cooperation and collaboration helped to kind of promote science on a broader scale and so we do take our students on-site visits to biotech companies and see how local biotech companies also incorporate some of the same things that we're doing in our classroom and this is pictures an example of one of those and we also do collaborations with local biotech companies as well not in this class in particular at least as of yet so to close I just wanted to kind of show you a little bit about how I carry out the assessment of this course again it's relatively similar I think to what was presented at the sim bio workshop that I attended I do add in a few extra things with a math packets that I mentioned and I do do a final lab poster as I mentioned in a presentation the lab meeting does come into play and this research habits of mind come into play but I used the final lab benchmark that was similar to those that were presented when I went to the workshop at OBU and the final lab part that was lab report that was very similar to those that were used at OBU so otherwise overall I really have a heavy component of my overall course grade that is made up from this laboratory components about 40% this made up of the laboratory component because of how much time I students spend in the lab as I mentioned at the very beginning students are in the lab for two hours two times a week but when it comes down to it students spend much more time in the lab I would say many students will come in to set up cultures in between so my lab runs Tuesdays and Thursdays two hours a day so on a Monday or Wednesday students will come in pick colonies to set up cultures or sometimes set up digester things like that so on average I would say most students spend about six hours a week total so two extra hours a week in the lab I set that up ahead of time there are lab teams that don't do that and they are certainly successful but there are many lab teams that you spend extra time so if you have any questions at all feel free to contact me my contact information is here my email and certainly feel free to utilize or edit any of the resources or handouts that I will post in the folder I will post them all as MS or documents as least as many as I have to post that way and I'm certainly happy for you to take any and all of them edit them as you please and certainly let me know if you have any questions it was great to be able to provide this video for you guys I hope that you have a wonderful workshop take care

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How do you make a document that has an electronic signature?

How do you make this information that was not in a digital format a computer-readable document for the user? " "So the question is not only how can you get to an individual from an individual, but how can you get to an individual with a group of individuals. How do you get from one location and say let's go to this location and say let's go to that location. How do you get from, you know, some of the more traditional forms of information that you are used to seeing in a document or other forms. The ability to do that in a digital medium has been a huge challenge. I think we've done it, but there's some work that we have to do on the security side of that. And of course, there's the question of how do you protect it from being read by people that you're not intending to be able to actually read it? " When asked to describe what he means by a "user-centric" approach to security, Bensley responds that "you're still in a situation where you are still talking about a lot of the security that is done by individuals, but we've done a very good job of making it a user-centric process. You're not going to be able to create a document or something on your own that you can give to an individual. You can't just open and copy over and then give it to somebody else. You still have to do the work of the document being created in the first place and the work of the document being delivered in a secure manner."

How do i insert an electronic signature into a word document?

How do I sign a text file with a text editor? How do I convert an .rtf, .otf, or .woff file to a proper .doc format? How do I edit an .doc file using an application like MS Word? How do I save an .doc or .rtf file in Adobe Illustrator format? Can I import a .doc, .rtf, or .otf file in Microsoft Publisher? How do I convert WordPerfect (.doc), MS Word (.doc), OpenOffice/LibreOffice/Adobe Acrobat (.odt). How do I import a file using MS Outlook? How do I import a Microsoft Office Document? I'm having trouble saving a document (how do I find a particular document in the archive? what does that mean? what does it mean to add something to a file or folder in Exchange? I'm having problems saving documents in Microsoft Office, is there any way I can export or save these documents? If so, what settings would make the file most helpful to me? I'm having problems saving a file in Microsoft Office (Exchange). Is it possible to find out how a file is saved? I'm trying to get a document to print but cannot find the printer I want to use. How do I set up the printer and find it on the network? Do you have a tool that shows me which Exchange servers can access the Exchange Online folder structure? What are the differences between the Exchange 2003, Exchange 2004, Exchange 2007, Exchange 2010 and Exchange 2013? Can you describe the differences between the three Exchange Server versions? If an Exchange user has multiple email addresses, how can I change their email...

How to sign and sends pdf document online?

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