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Quick guide on how to complete in transcription rna polymerase splits the two halves of a strand of dna
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What is the full form of DNA and RNA?Nuciec acids are biomolecules that plays a vital role in the existence of life. Nucleic acids are polymer of nucleotides joined together through phosphodiester bond. A nucleotide is composed of three components : a nitrogenous Base (Adenine,Guanine, Cytosine, Thymine or Uracil), pentose sugar (ribose or deoxyribose) and a phosphate molecule. There are two types of nucleic acids : Deoxyribonucleic acid or DNA and Ribonucleic Acid or RNA.DNADeoxyribonucleic acid or DNA is a double stranded molecule responsible for carrying all the genetic information. 99.9% DNA of every human being is same while it is the rest 0.1% that differs. DNA is present in a highly supercoiled form known as chromosomes. These chromosomes carry genes that encode genetic information. Human beings have 23 pairs of chromosomes (22 autosome and 1 sex chromosome). DNA is transcribed into mRNA which further gets translated into proteins. This is known as LAW OF CENTRAL DOGMA.DNA mRNA ➡️ ProteinsRNARibonucleic Acid or RNA is a single stranded molecule which plays an important role in gene expression. Many viruses have RNA as their genetic material. There are three types of RNAMessenger RNA or mRNA(carries information from DNA to ribosomes)Transfer RNA or Tina (transfers specific amino acid to site of protein synthesis)Ribosomal RNA or rRNA(catalytic component of ribosomes)Difference between DNA and RNADNA has nitrogenous bases Adenine, Thymine, Guanine and Cytosine while RNA has Adenine, Guanine Cytosine and Uracil. Base pairing always occurs as G-C and A-T in DNA while G-C and A-U in RNA.Pentose sugar in DNA is deoxyribose while in RNA it is Ribose.3. DNA is double stranded while RNA IS a single stranded molecule.4. DNA is self replicating while RNA isn't.5. DNA is short of one oxygen in its 2′ position which makes it more stable than RNA.
Could there be alien (i.e., non DNA/RNA, independently originated) forms of life on Earth, and if so, how we would find them?It would indeed be novel if we discover xeno-life that does not use DNA or RNA as its genetic code. Though certainly scientifically possible, no alternative genetic code has so far been proposed to my knowledge. Note that DNA is very well-suited for its role, being a stable and highly efficient molecule for storage of information (1 gram = 700 TB of data).Could life exist without a genetic code? Well, Edward Trifonov defines life as "self-reproduction with variations". Viruses, and perhaps even prions, would be included in such a definition, but what about a computer virus with a mutagenic engine or the hypothetical nano-bot that self-replicates? Apparently, some proponents of the Shadow biosphere think that even Desert varnish could be alive.The point is that, without knowing what non-DNA/RNA life is like, we might not really know how to detect it unless it steps out of a flying saucer or some blob, goo or dust-cloud from space starts consuming our solar system.Paradoxically, it might be easier to detect DNA-based xeno-life.All life on earth share certain biochemical features which strongly suggest descent from the LUCA (Last Universal Common Ancestor). There are potential biochemical anomalies that, if detected, would imply either a different evolutionary path from abiogenesis (life from non- life), genetically-engineered life, or life of non-earth origin. To name a few:1. Violation of Homochirality: all earth-life use only left-handed amino acids and right-handed nucleotides and sugars.2. Xeno-Nucleotides: the use of nucleobases other than A, G, C & T.3. Different mechanism for transcription (DNA to RNA).4. Different mechanism for translation (RNA to proteins)5.Different energy-carrier (other than ATP and NADPH)Of course, these clues will probably be absent if all life in the cosmos shared the same origins.
What happens to all of the signNow forms you fill out for immigration and customs?Years ago I worked at document management company. There is cool software that can automate aspects of hand-written forms. We had an airport as a customer - they scanned plenty and (as I said before) this was several years ago...On your airport customs forms, the "boxes" that you 'need' to write on - are basically invisible to the scanner - but are used because then us humans will tend to write neater and clearer which make sit easier to recognize with a computer. Any characters with less than X% accuracy based on a recognition engine are flagged and shown as an image zoomed into the particular character so a human operator can then say "that is an "A". This way, you can rapidly go through most forms and output it to say - an SQL database, complete with link to original image of the form you filled in.If you see "black boxes" at three corners of the document - it is likely set up for scanning (they help to identify and orient the page digitally). If there is a unique barcode on the document somewhere I would theorize there is an even higher likelihood of it being scanned - the document is of enough value to be printed individually which costs more, which means it is likely going to be used on the capture side. (I've noticed in the past in Bahamas and some other Caribbean islands they use these sorts of capture mechanisms, but they have far fewer people entering than the US does everyday)The real answer is: it depends. Depending on each country and its policies and procedures. Generally I would be surprised if they scanned and held onto the signNow. In the US, they proably file those for a set period of time then destroy them, perhaps mining them for some data about travellers. In the end, I suspect the "signNow-to-data capture" likelihood of customs forms ranges somewhere on a spectrum like this:Third world Customs Guy has signNow to show he did his job, signNow gets thrown out at end of shift. ------> We keep all the signNows! everything is scanned as you pass by customs and unique barcodes identify which flight/gate/area the form was handed out at, so we co-ordinate with cameras in the airport and have captured your image. We also know exactly how much vodka you brought into the country. :)
Is it possible to label a DNA molecule using a radioactive sulphur? Is yes how? And if no why?I personally think that it is not possible as because the DNA molecule does not contain any sulphur atom which can be replaced by the radioactive sulphur molecule.
How does a zygote transcribe its DNA initially given that RNA Polymerase is a protein and has to be transcripted and translated to form? Would it use cytoplasmic egg RNA Polymerase then change to use the zygote RNA polymerase?The ovum is a fully functional cell packed with all the proteins needed to get embryogenesis started after fertilization. These proteins would have been synthesized during the maturation process of the ovum.The zygote and its progeny would produce more polymerase as needed as it grew, with fundamentally there is no difference between “egg polymerase” and “zygote polymerase”. It is still the exact same gene, and in the beginning it would even be the exact same stretch of DNA.
How can one break the capsid of a bacteriophage to release its RNA and DNA?Do you mean to ask, how can one isolate nucleic acid from the bacteriophages?Well, there are various kits available to help you in this process. However if you must use a manual protocol , the first step would be to dialyze the isolated viral population against the dialysis buffer to rid of inonic contamination.to lyse the viral particles you can use (EDTA : SDS : proteinase K) cocktail in the concentration of (20mM : 50ug/ml : 0.5%) and incubate for an hour.This can then be followed by the usual phenol:chloroform extraction, precipitation using ammonium acetate (for DNA) and lithium acetate (for RNA) and then final wash using 70% EtOH.You should get a yield of around 500 ng/ml using this manual protocol.Hope it helps!
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People also ask
What are DNA probes and how do they work?Gene probes are small, single-stranded fragments of DNA that hybridize to target DNA sequences in a sample. Tagged with a label like color or fluorescence, they allow researchers to identify a specific sequence of DNA in a mixture. First, the DNA sample is heated to separate the DNA strands, then the probe is applied.
What is probe labeling?Probe labelling. ... NUCLEOTIDE PROBES \uf0a2 A probe is defined as a single stranded piece of DNA, labelled (either with radioisotope or with non-radioactive label), the nucleotide sequence of which is complementary to the target DNA.
What is a probe used for?Medical Definition of Probe Probe: 1: In surgery, a probe is a slender flexible rod with a blunt end used to explore, for example, an opening to see where it goes. 2: In molecular genetics, a probe is a labeled bit of DNA or RNA used to find its complementary sequence or locate a particular clone.
What is Nucleic Acid Labeling?Chemical methods for nucleic acid labeling Aldehyde groups that are created in solutions with periodates are spontaneously reactive towards amine-containing surfaces or molecules. Thus, periodate oxidation of RNA is a common chemical method for nucleic acid labeling.
What is Labelling of DNA?Product Listing Product Overview. Nucleic acids are readily labeled with tags that facilitate detection or purification. A variety of enzymatic or chemical methods are available to generate nucleic acids labeled with radioactive phosphates, fluorophores, or nucleotides modified with biotin or digoxygenin for example.