Idexx Mpn Table Form
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People also ask
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What is the principle of Colilert?
Principle: The Colilert-18 test uses proprietary Defined Substrate Technology (DST) nutrient indicators ONPG and MUG to detect coliforms and E. coli. Coliforms use their β-galactosidase enzyme to metabolise ONPG and change it from colourless to yellow.
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What is the function of the quanti-tray sealer?
The Quanti-Tray Sealer PLUS is used with Quanti-Tray and Quanti-Tray/2000 to quantify bacterial testing results for coliforms, E. coli, enterococci and Pseudomonas aeruginosa. The new system: Warms up 80% faster.
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What is a quanti-tray?
Quanti-Tray indicates the quantity of coliform bacteria by the number of wells that turn yellow or magenta. Fluorescent wells indicate the quantity of E. coli. A simple MPN table converts well counts to a Most Probable Number. Results are both presumptive and confirmatory — no additional testing is required.
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What is the shelf life of Colilert?
What is the shelf life of the Colilert test? The shelf life of the Colilert test is up to 12 months from the date of manufacture for the snap pack formats (100 mL) and up to 15 months from the date of manufacture for glass-tube formats (10 mL).
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What is colilert quanti-tray?
ISO Standard: The Colilert-18/Quanti-Tray method is a worldwide ISO standard for detecting total coliforms and E. coli in water (ISO 9308-2:2012). 18-hour Confirmed Result: Samples incubated overnight are ready to read early the next day.
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What is the difference between Colilert and Colilert-18?
Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35° C, rather than the 24 h required for the Colilert® test, and is recommended for marine water samples.
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How does Colilert 18 work?
How it works. The Colilert-18 test uses a proprietary Defined Substrate Technology (DST) nutrient indicators ONPG and MUG to detect coliforms and E. coli. Coliforms use their β-galactosidase enzyme to metabolize ONPG and change it from colorless to yellow.
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Why do coliforms turn yellow in the quanti Tray?
Two nutrient-indicators, ONPG and MUG, are metabolized by the coliform enzyme β-galactosidase and the E. coli enzyme β-glucuronidase, respectively. As coliform organisms grow during incubation at 35.0 ± 0.5°C, they use β-galactosidase to metabolize ONPG and change it from colourless to yellow.
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What is the incubation time for total coliform?
Incubate the inverted petri dish at 35 ± 0.5 °C (95 ± 0.9 °F) for 22–24 hours.
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What is the quanti-tray method?
With Quanti-Tray, a 100 mL sample is divided into 51 wells. Then the Standard Methods' Most Probable Number (MPN) approach is used to determine the number of bacteria in the original sample. Automation minimizes hands-on time and maximizes throughput (up to 4 samples per minute).
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