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Stanford Antibiotics PDF Form

Stanford Antibiotics PDF Form

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So what we ended up building was basically a Petri dish, except that it's 2 feet by 4 feet. And the way we set it up is that there are nine bands, and at the base of each of these bands we put a normal Petri-dish-thick agar with different amounts of antibiotic. On the outside there's no antibiotic; just in from that there's barely more than the E. coli can survive; inside of that, there's 10 times as much; a 100 times; and then finally the middle band has a thousand times as much antibiotic. And then across the top of it we pour some thin agar that bacteria can move around in. The background is black because there's ink in it, and the bacteria appear as white. First you see they spread in the area where there's no antibiotic up until the point that can no longer survive. Then a mutant appears on the right. It's resistant to the antibiotic. It spreads until it starts to compete with other mutants around it. When these mutants hit the next boundary, they too have to pause and develop new

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