
Citrate Buffer Table Form


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People also ask
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What is sodium citrate buffer preparation and why is it important?
Sodium citrate buffer preparation involves mixing sodium citrate with a specific pH of distilled water to create a stabilizing solution. This buffer is crucial in biochemical experiments because it helps maintain the pH levels during reactions and optimizes enzyme activity.
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How do I prepare a sodium citrate buffer solution?
To prepare a sodium citrate buffer solution, you typically dissolve sodium citrate in distilled water and adjust the pH using citric acid or sodium hydroxide as needed. It's essential to follow the proper concentrations depending on your specific experimental requirements for effective sodium citrate buffer preparation.
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What are the common applications of sodium citrate buffer?
Sodium citrate buffer is widely used in biological research, particularly in enzyme assays and DNA extraction protocols. Its ability to maintain a stable pH makes sodium citrate buffer preparation a preferred choice for experiments requiring precise conditions.
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Are there any alternatives to sodium citrate buffer?
Yes, there are several alternatives to sodium citrate buffer, such as phosphate buffers and Tris buffers. However, sodium citrate buffer preparation is often favored for its simplicity and effectiveness in certain biochemical applications.
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What is the cost of purchasing pre-made sodium citrate buffer solutions?
The cost of pre-made sodium citrate buffer solutions can vary based on the supplier and volume purchased, typically ranging from $50 to $200 per liter. For frequent users, preparing your own sodium citrate buffer may be more cost-effective over time.
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Can I use sodium citrate buffer for tissue culture applications?
Yes, sodium citrate buffer can be used in tissue culture applications to maintain pH stability during various procedures. Proper sodium citrate buffer preparation helps ensure optimal conditions for cell growth and maintenance.
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What resources are available for understanding sodium citrate buffer preparation?
Many online resources, including research articles and laboratory manuals, provide detailed guidance on sodium citrate buffer preparation. Websites dedicated to laboratory techniques also offer practical tips and protocols tailored to different applications.
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