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Citrate Buffer Table Form

Citrate Buffer Table Form

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prepare a hundred milliliters of a 0.1 molar buffer and like to illustrate how to do this in the lab let's let's take citrate as an example citrate has three PKS 3.06 four point seven four and five point four O so we could buffer at any of those three PHS let's choose the four point seven four four as an example and we see the molecular weight is given here as two hundred ninety four point one grams per mole this is for the trisodium hydrate material which we have here in the lab so citrate as we'll learn is a tri carboxylic acid so it has that's why it has three PK's and to calculate how much sodium citrate we would need to prepare our solution we can come over here to the board and calculate grams of trisodium citrate it has water in it but the molecular weight is to 94.1 grams per mole and we're making a point one mole per liter or 0.1 over a thousand milliliters solution and we need a hundred mils of it so if we multiply we get two point nine four grams of sodium citrate are requir

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