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Kiwi ACE R Version a V1f DOC Ftdrg Form

Kiwi ACE R Version a V1f DOC Ftdrg Form

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[Music] so in the context of avid one of the things I'm working on is developing a uh cellular thermal shift uh setsa Mass spectrometry based assay and the general idea here is that um when proteins are heated um they'll Aggregate and then they'll become less soluble and um we can take advantage of that in the context of drug binding um so if you have a drug that is bound to a protein that protein will become more stable um and then when you subject your proteins to a heat gradient um You can compare between for example uh like control samples and treated samples and we look for a shift in What's called the um melting point or the point at which proteins are 50% aggregated in solution and the idea is that if a drug is bound to a specific protein it's going going to have um this like melting point at a higher temperature because it's more stable so when we analyze the data we can look for these shifts in we call the Melting curves of the d

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