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Confocal Cif Form

Confocal Cif Form

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[Music] hey everyone quick bar chemistry basics here let's talk about confocal microscopy confocal microscope was invented because of drawbacks of fluorescence microscope so what are the drawbacks of fluorescence microscope in fluorescence microscopy it uses a high-intensity UV light as the samples are continuously exposed to high-intensity UV light it causes photo bleaching as all the fluorescence molecules of sample a bell and below the focal plane gives fluorescence the image obtained is blurred so to solve these problems a confocal microscope was invented a confocal microscope is nothing but a modified fluorescence microscope with two major modification number one it uses a laser light instead of mercury arc lamp as a source of light and number two images are taken with a digital camera with a pinhole what's the function of pinhole pinhole allows light of only one focal plane to be focused on the digital camera the light rays coming from a bow and below the focal plane are canceled

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