
MLG 8 09 Isolation and Identification of Listeria Monocytogenes from Red Meat, Poultry and Egg Products, and Environmental Sampl Form


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People also ask
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What foods should you avoid if you have Listeria monocytogenes?
Past listeriosis outbreaks in the U.S. have been linked to raw, unpasteurized milks and cheeses, ice cream, raw or processed vegetables, raw or processed fruits, raw or undercooked poultry, sausages, hot dogs, deli meats, and raw or smoked fish and other seafood.
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How do you identify Listeria monocytogenes?
The diagnosis of L. monocytogenes requires a culture of the bacteria from the blood, cerebral spinal fluid, or placental fluid. Once in the lab, Listeria species grows on a special type of agar called Meuller-Hinton agar. Culture will reveal gram-positive rods with colonies that are beta-hemolytic.
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How is Listeria monocytogenes detected and isolated?
Identification of the L. monocytogenes was done ing to the standard microbiological tests such as catalase, oxidase, β haemolysis, motility test at 25°C, the CAMP assay, and acid production from xylose and rhamnose (17).
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What are the typical sources and methods of transmission of Listeria?
Listeria can be spread to people by several different methods. Eating food contaminated with the bacteria, such as through raw (unpasteurized) milk or contaminated vegetables, is often a source for cases. The bacteria may be passed from mother to fetus during pregnancy or directly to the newborn at the time of birth.
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What media is used for isolation of Listeria monocytogenes?
Hemolytic ceftazidime lithium chloride agar (HCLA) has been developed as a selective and differential plating medium for the isolation of Listeria monocytogenes from fishery products. Selectivity is based upon lithium chloride, colistin methane sul- fonate, and ceftazidime.
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How do you test for Listeria monocytogenes?
Current methods for identification of L. monocytogenes rely on physiological and biochemical methods. These include Gram stain morphology, catalase, motility, beta hemolysis on blood agar and oblique illumination of colonies on blood free agar (6).
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How do you isolate Listeria?
McBride and Girard (47) observed that incubation of the sample in tryptose phosphate broth containing 10 ppm of Furacin at 37°C for 48 h followed by plating on McBride Listeria Agar was an effective method to isolate L. monocytogenes from experimental mixed cultures.
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What are the traditional methods for isolation of Listeria monocytogenes?
3.1 Detection of L. monocytogenes Weigh 25 g of analytical portions of solid food or 25 mL liquid foods into a sterile plastic bag. ... Incubate for 24 h at 30 °C. After incubation transfer 0.1 mL of the pre-enrichment broth culture to the 10 mL of enrichment broth medium (Fraser). Incubate for 24 h at 37 ºC.
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