CDNA Sequence and Heterologous Expression of Monomeric Spinach Biochemj Form

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[Music] the central dogma biology is that dna makes rna which makes protein since the 1970s heterologous protein expression has been achieved using recombinant dna technology identification of the promoter region of dna where rna polymerase binds in order to transcribe dna into rna was a critical step in understanding this process another important discovery was the identification of the operator region of dna where proteins that regulate gene expression bind these discoveries led to the development of systems that can incorporate promoters operators and associated dna elements into a circular piece of dna called a plasma to drive protein expression plasmids can be replicated by the cell's native dna polymerases and are easily isolated from or transform into e coli while there are many types of promoters the three used most commonly for protein expression in e coli are the lack era and trip promoters some of these promoters rely on additional short dna sequences known as operators to r