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CDNA Sequence and Heterologous Expression of Monomeric Spinach Biochemj Form

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hello friends welcome to bmh learning in this video we will talk about serial analysis of gene expression also called ch sage uses mrna from particular sample to create cdna fragments which are then amplified and sequenced using high throughput sequencing technology principle behind sage is based on tags which can identify the original transcript and rapid sequencing of chains of tags linked together the procedure essentially simplifies sequencing by linking the cdna segments together in a long chain the resulting analysis gives a snapshot of the transcript term of the sample including the identity and abundance of each mrna how it works step one cdna is synthesized from poly a plus rna using the biotin oligodd step 2 cdna is bound via biotin to strepta between microbeads and is cleaved with restriction enzymes known as anchoring enzymes this frees it from beads step 3 two different linkers containing a 5 base pair recognition site for a type 2 restriction enzyme called tagging enzyme

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CDNA Sequence and Heterologous Expression of Monomeric Spinach Biochemj Form

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How to create an eSignature for the cdna sequence and heterologous expression of monomeric spinach biochemj

How to create an electronic signature for a PDF online

How to create an electronic signature for a PDF in Google Chrome

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How to create an e-signature right from your smartphone

How to create an e-signature for a PDF on iOS

How to create an e-signature for a PDF on Android

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