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Retroviral Expression of Alternatively Spliced Forms of Rat Fibronectin

Retroviral Expression of Alternatively Spliced Forms of Rat Fibronectin

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last module we have it also has a tutorial component we're gonna do it's really only meant as kind of a brief introduction to the the topic of isoform discovery and alternative expression analysis anyways that is a very complex area with a lot of tool development that's still underway and the tools that we're going to use here they're trying to do something very elaborate and they do kind of an okay job at it but it's an area that I think still needs a lot of development so it's a challenging problem to actually do isoform specific expression analysis with RNA seek data and I'll kind of explain why that why that is during this this lecture so really what we're gonna do in terms of the the hands-on component here is use cufflinks again but in a different mode that's a little bit more geared towards isoform discovery and abundance estimation but both of these modes still require a reference genome sequence so

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