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hello and welcome to my today's presentation i'm lisa mize and i'm a phd student in doug mckay miller's lab at the mdc in berlin today i'm talking about patch perfect's match a method for automated 3d search of neuronal morphologies and light microscopy data the work is based on two large projects done at generia howard youth medical institute and aims at bringing them together so the first one is the fly em project where they released the hemi frame connectome here they imaged one fruit fly brain with electron microscopy and traced all neurons and synapses on it on the other hand there's the flylite project which aims at providing a large resource of transgenic lines for targeted neuron manipulation and imaging with light microscopy data on the left side here you can see the full expression pattern of such a line and the two right images are multi-color flip out acquisitions which is a storage labeling technique to only show a random subset of the full expression pattern and to have a

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