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Teacher's Guide PBLU Transformation

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in this video you will learn how to perform a bacterial transformation eoli hb101 bacteria will be transformed with P glow plasmid using a calcium chloride transformation to start this activity you will need an lb auger starter plate streak for single colonies of eoli hb101 and two micro centrifuge tubes each with 250 microl of 05 molar calcium chloride or transformation solution one of the tubes should be labeled plus and the other minus both tubes should be kept on Ice flame an inoculation Loop and allow it to cool you can light ly touch the loop on an empty spot on the starter plate to make certain the loop is cool then gently scrape one large or two small colonies from the surface of the starter plate so that they can collect on the loop transfer the loop with bacteria to the plus tube and swirl it in the transformation solution to disperse the bacteria close the tube and place it back on Ice Flame the loop and repeat the process to add bacteria to the minus tube using a micropipet

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Teacher&#039;s Guide PBLU Transformation

How it works

Quick guide on how to complete teacher39s guide pblu transformation

Easily prepare teacher39s guide pblu transformation on any device

How to edit and electronically sign teacher39s guide pblu transformation effortlessly

Create this form in 5 minutes or less

Video instructions and help with filling out and completing Teacher&#39;s Guide PBLU Transformation

Instructions and help about Teacher&#39;s Guide PBLU Transformation

Create this form in 5 minutes!

How to create an eSignature for the teacher39s guide pblu transformation

How to create an electronic signature for a PDF online

How to create an electronic signature for a PDF in Google Chrome

How to create an e-signature for signing PDFs in Gmail

How to create an e-signature right from your smartphone

How to create an e-signature for a PDF on iOS

How to create an e-signature for a PDF on Android

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Teacher's Guide PBLU Transformation

Video instructions and help with filling out and completing Teacher's Guide PBLU Transformation

Instructions and help about Teacher's Guide PBLU Transformation

Get more for Teacher's Guide PBLU Transformation

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