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Teacher's Guide PBLU Transformation
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People also ask
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Is pBluescript a plasmid vector?
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. -
What are the key steps in the transformation procedure?
The four key steps in bacterial transformation are 1) Competent cell preparation, 2) transformation of cells, 3) cell recovery, and 4) cell plating. -
What does pBLU do?
pBLU carries the gene for β-galactosidase (also known as lacZ). This enzyme catalyzes the breakdown of lactose as a food source. It can also degrade an artificial substrate called x-gal, which turns BLUE when it is broken down by β-galactosidase. -
How big is the pBLU plasmid?
pBLU is a commercially produced bacterial plasmid that contains genes for resistance (beta lactamase and beta galactosidase). It is often used in conjunction with an -susceptible E. coli strain to teach students about transformation of eubacteria. It is 5,437 base pairs long. -
What is an example of a plasmid?
Plasmids are also found in higher organisms such as yeast and fungi. The 2 micron circle of yeast (discussed later) is a well-known example that has been modified for use as a cloning vector. Most plasmids are circular, made of DNA, and much smaller than chromosomes. -
Is pBLU a plasmid?
pBLU is a plasmid that contains the gene for beta-galactosidase, that when expressed and combined with X-Gal is used f as a visual indicator of a functional lacZ transformation. pFLO contains a type of Green Fluorescent Protein (GFP) gene that when expressed by E. coli will make them glow under blue LED or UV light.
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