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Registration Form for Recominant Dna Experiments Personnel Update

Registration Form for Recominant Dna Experiments Personnel Update

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early in 1973 not even a year after the discovery of the appropriate tools we talked about Stanley Cohen along with a bunch of other researchers carried out the first experiment in gene technology researchers chose a small plasmid named after Cohen's initials B s c-101 which occurs naturally in large quantities in bacterial cells this plasmid carries the T C gene that confers resistance to antibiotic tetracycline the plasmid was mainly chosen because it contains just one g-a-a-t-t-c base sequence which we have already seen is the restriction site for the restriction endonuclease eco r1 which cleaves between the G and K bases within this sequence if there was more than one of this sequence the plasmid ring would have been cut into several pieces rather than just one in order to identify the bacteria containing the foreign gene it was crucial that this restriction process did not disable the antibiotic resistance properties as well eco r1 originates from ecoli and the cutting pro

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