
Pipetting of Samples and Reagents Must Be Done as Quickly as Possible Funakoshi Co Form
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People also ask
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How does temperature affect pipetting?
Temperature has many effects on pipetting accuracy. The factor that has the greatest effect is the temperature difference between the delivery device and the liquid. The air gap (dead air volume) between the liquid surface and the piston experiences thermal expansion effects unique to the case.
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What are the factors affecting pipette accuracy?
Factors Affecting Pipette Accuracy Temperature. Temperature has many effects on pipetting accuracy. ... Density. The density (mass/volume ratio) affects the liquid volume that is aspirated into the tip. ... Altitude. Geographic altitude affects accuracy through air pressure.
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What are the sources of error when using a pipette?
Forcing the solution out of the pipet causes too much to be delivered. Using a dirty pipet causes too little or contaminated solution to be delivered. Leaving little droplets behind on the walls (except for the small amount in the tip) causes too little solution to be delivered.
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How do you avoid contamination of samples or reagents in pipetting?
Use Safe-Cone Filters or Filter Tips Safe-Cone filters protect the pipette from contamination and volatile liquids. It is recommended to change the filters daily (after 50 to 250 pipetting) and always in case of overaspiration. Filter tips gives the best protection for both the samples and the pipette.
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What are three possible sources of error in this lab?
Physical and chemical laboratory experiments include three primary sources of error: systematic error, random error and human error. These sources of errors in lab should be studied well before any further action.
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What are three possible sources of error in the use of a pipette?
If you have questions about how to carry out your experiment, ask our experts to advise you throughout the process. Mistake #1: not assessing the viscosity of the sample. ... Mistake #2: DISPENSING LIQUID REAGENTS TOO FAST. ... MISTAKE #3: PIPETTING DIFFERENT SAMPLES WITH THE SAME TIP.
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What are the rules for pipetting?
Wait about one second for the liquid to flow into the tip. Put the pipette—held at 10–45 degrees—against the wall of the receiving chamber, and smoothly depress the plunger to the first stop. Wait one second and then depress the plunger to the second stop. Slide the tip up the vessel wall to remove the pipette.
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What is the wrong use of a pipette?
Pipetting at incorrect angles. Your pipette should be vertical when aspirating. Working too quickly while pipetting is another common pipetting error. Let the liquid settle into the pipette tip for a second or two after aspirating.
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