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Booking Sheet for the NextSeq 550 Form DOCX

Booking Sheet for the NextSeq 550 Form DOCX

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Instructions and help about Booking Sheet For The NextSeq 550 Form docx

hello everybody welcome to a tutorial on how to load a nexus 5 hundred by Illumina so make sure you taught your chemistry before in the know water bath takes around an hour once you did that you have to gently rock it five times to mix all the reagents after us check if all the regions are completely taught if that is the case you to take an empty a Piper tip and poke a hole into well number 10 where you load library make sure to make the hole as big as possible afterwards take your library and pipe at one point three milliliter into well number 10 now be careful not to introduce any air bubbles or touched aluminum foil with a pipette rock on the table gently once or twice and put it into the next seek afterwards you can take your buffer cartridge and load it into the flapper spot make sure the garbage is in the emptied and close the door again now I take your fresh flow cell out of the casing should be in room temperature for about half an hour and check it against a light background

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