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96 Well Plate Template  Form

96 Well Plate Template Form

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Instructions and help about 96 well plate template printable

After you've successfully used the hemocytometer and come up with an accurate cell number, you and your instructor will come up with a calculation to create a stock solution for seeding a 96-well plate. Once that stock has been created you'll pipette out 100 microliters of that Cell-DMEM stock into each of your designated wells as seen in the lab handout so I will show you guys how to properly do that as follows so make sure you select the right micropipetter and have it set to 100 uL, and use the right filtered tips and set this aside (the plate lid). what I always do is I hold my stock in one hand with that same hand I tilt it [the plate] forward so I can see the empty wells otherwise it's very hard to differentiate which wells you've pipetted into and which ones you haven't. Make sure to properly pipette up and down your cells and your cell stock to make sure that your cells are properly distributed when pipetting out ensure that you look into your tip that you don't have any bubble

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