Bd Fluorochrome Chart Form
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People also ask
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What is the most widely used fluorochrome?
FITC (Fluorescein): FITC is the most widely used form for conjugation to antibodies and proteins, but other derivatives are available. FITC gives a decent fluorescent signal in the green region of the spectrum. Though, precaution must be taken when using FITC as it is sensitive to pH changes and photobleaching.
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What are the uses of fluorochrome?
Fluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength.
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What is fluorochrome used for?
As a result of their intense fluorescence, these fluorochromes are useful for measurements that require high sensitivity, such as cellular antigens that are expressed at low epitope density.
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How do I choose fluorochromes?
Choose fluorochromes with emissions having the least spectral overlap. Generally, the further apart the maximal emissions, the less overlap there will be, but this is fluorochrome-dependent. There are several tools available on the to help you analyze fluorochrome spectra.
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What is the most widely used fluorochrome?
FITC (Fluorescein): FITC is the most widely used form for conjugation to antibodies and proteins, but other derivatives are available. FITC gives a decent fluorescent signal in the green region of the spectrum. Though, precaution must be taken when using FITC as it is sensitive to pH changes and photobleaching.
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What is the difference between BV510 and V500?
BD Horizon™ Brilliant Violet™ 510 (BV510) (Exmax 405 nm/Emmax 510 nm) is a polymer-based dye that is brighter than BD Horizon™ V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
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What is a fluorescent dye test used to check for?
This is a test that uses orange dye (fluorescein) and a blue light to detect foreign bodies in the eye. This test can also detect damage to the cornea.
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What is the difference between fluorescent and fluorochrome?
A fluorochrome refers to any fluorescent dye used to stain cells or tissue for microscopic examination. A fluorophore refers to any molecule or functional group which is capable of fluorescence. Therefore, all fluorochromes are fluorophores, but not vice versa…
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