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Promega Dna Extraction Kit Form

Promega Dna Extraction Kit Form

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Instructions and help about promega dna extraction kit

Hi! Today we'll be extracting plasmid DNA from bacteria, it's a protocol is usually called a miniprep It's called minipreps because we will be extracting DNA from a small volume of bacterial culture. You can also do midipreps and maxiprep from bigger volumes, but we won't discuss it today So to start you have to start a bacterial culture for this we need a culture tube and some media To keep it sterile we will be working around the source of heat I will now pour some media into the cell culture tube About five mL is enough We also need to add antibiotics to the medium. In this case it's kanamycin The antibiotic is needed to prevent other contaminants from growing this way only our plasmid our bacteria containing our plasmid will grow in this medium And now we have to pick one colony one single colony from a plate. We also are doing this in sterile environment So now all we have to do is to label our tube and put it to incubate at 37 degrees overn

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