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4 as Protocol Worksheet  Form

4 as Protocol Worksheet Form

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Instructions and help about 4 As Protocol Worksheet

[Music] before beginning this protocol students must fill out the worksheet provided on the last page of this document this will allow students to calculate the appropriate volume of reagents to mix in the creation of a master cocktail the purpose of a cocktail is to mix all the common re agents in a multi- sample procedure to avoid excess pipetting and potential errors in this example of protocol 2A four students are amplifying the actin and 3 Gene from all four of their DNA samples this information is filled out on the left hand side of the [Music] worksheet on the right hand side students use the equations to determine the number of tubes needed and the multiplier for reagent volumes in addition to the 4 d samples a negative control sample is always included therefore five tubes are needed for the PCR reaction in the creation of the cocktail pipetting error is accounted for by adding another sample therefore the multiplier for this group is [Music] six each row of table one represen

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