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People also ask
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Which technique is used to detect proteins?
Western blot is often used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein.
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How are proteins localized within a cell?
Signal sequences within the protein itself will interact with recognition proteins at the mitochondrion to determine the protein's final location. A mitochondrial proteins' final destination (outer membrane, intermembrane space, inner membrane, or matrix) will be determined by its particular array of signal sequences.
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What is a method used to visualize the location of a protein in a cell?
Visualization of protein bands is carried out by incubating the gel with a staining solution. The two most commonly used methods are Coomassie and silver staining. Silver staining is a more sensitive staining method than Coomassie staining, and is able to detect 2–5 ng protein per band on a gel.
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How does the cell detect where proteins should go?
Specific signal sequences, small sections of proteins that act like “post codes”, are vital for delivery to the correct location and proper insertion into the membrane. They are detected by molecular sorting machines that deliver the protein to its destination.
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What is the subcellular localization of proteins?
Subcellular localization refers to the identification of where a protein is located within a cell, which provides insights into its potential functions.
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How to find protein location?
Experimentally determining the subcellular localization of a protein can be a laborious and time consuming task. Immunolabeling or tagging (such as with a green fluorescent protein) to view localization using fluorescence microscope are often used.
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What method can be used to localize proteins in cells?
Techniques such as immunofluorescence and high-resolution confocal microscopy have enabled the visual estimation of protein localization within a single cell [20], [21], [22], [23], [24].
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What are the methods of protein localization?
Experimentally determining the subcellular localization of a protein can be a laborious and time consuming task. Immunolabeling or tagging (such as with a green fluorescent protein) to view localization using fluorescence microscope are often used. A high throughput alternative is to use prediction.
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