Attune Fluorophore Selection Guide Form
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People also ask
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How do you choose which is the best fluorophore combination?
From experience, I would advise you to choose your fluorophores carefully. It is best to select the second fluorophore with a 2x longer delayed lifetime. This will help optimize your results.
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Which fluorophore to use?
As a rule, bright fluorophores should be conjugated to antibodies against low expressing cellular targets whereas fluorochromes of low brightness should be used for highly expressed proteins to avoid spillover and loss of resolution and sensitivity.
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How do you choose a fluorophore in a flow cytometry experiment?
Tips for Selecting Appropriate Fluorescent Dyes Choose the brightest set of fluorophores for your flow cytometer instrument configuration. Choose fluorophores to help minimize the spectral overlap. Reserve the brightest fluorophores for the weaker antibodies and vice versa.
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What is the flow rate of attune NXT?
The Attune NXT can reliably process data at sample flow rates up to 35,000 events/second.
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How to pick fluorophores for flow cytometry?
Tips for Selecting Appropriate Fluorescent Dyes Choose the brightest set of fluorophores for your flow cytometer instrument configuration. Choose fluorophores to help minimize the spectral overlap. Reserve the brightest fluorophores for the weaker antibodies and vice versa.
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How do you choose fluorophores?
Select fluorophores with high extinction coefficients ( ε ) One defining factor of a fluorophore's brightness is its extinction coefficient (a measurement of the probability of absorbing a photon of light); the higher the value of the extinction coefficient the brighter the fluorophore.
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How do you choose which is the best fluorophore combination?
From experience, I would advise you to choose your fluorophores carefully. It is best to select the second fluorophore with a 2x longer delayed lifetime. This will help optimize your results.
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What are the fluorophores used in flow cytometry?
Fluorescent proteins, such as green fluorescent protein (GFP), have become an integral tool for understanding protein expression in many scientific disciplines. Other fluorescent proteins, such as mCherry and yellow fluorescent protein, have also become widely used for flow cytometry analysis and cell sorting.
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