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Sequencing Rubric Form

Sequencing Rubric Form

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what do you call a collection of millions of DNA fragments sharing the same short sequences on the five Prime and three prime ends the answer is a Next Generation sequencing or NGS library today we are going to focus on the four basic steps of NGS Library preparation that can be broadly applied across different preparation [Music] methods a key step in the NGS workflow is preparing the input for sequencing known as creating a library an ngts library is a collection of similarly sized DNA fragments with known adapter sequences added to the five Prime and three prime EDS a library corresponds to a single sample and multiple libraries each with their own unique adapter sequences can be pulled and sequenced in the same sequencing run NGS Library preparation has four General steps DNA fragmentation or Target selection addition of adapter sequences size selection and final library quantification in QC let's take a look at our lab book the first step is DNA fragmentation or Target sel

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