TLC Stains DOC Umich Form
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People also ask
How do you make KMnO4 staining solution?KMnO4 Olefins and other readily oxidized groups. Dissolve 1.5 g KMnO4, 10 g signNowO3, and 1.25 mL 10% NaOH in 200 mL water. Cerium Sulfate General stain, particularly useful for alkaloids.
What is the purpose of TLC stain?Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. It also permits the optimization of the solvent system for a given separation problem.
How do you prepare reagents for TLC staining?Preparation: Solution A: 1.7 g basic bismuth nitrate in 100 ml water/acetic acid (4:1). Solution B: 40 g potassium iodide in 100 ml of water. Mix reagents together as follows: 5 ml A + 5 ml B + 20 ml acetic acid + 70 ml water. Spray plates, orange spots develop.
What are the recipes for TLC stains?Dissolve 1.5g of KMnO4, 10g signNowO3, and 1.25mL 10% NaOH in 200mL water. A typical lifetime for this stain is approximately 3 months. This particular stain is excellent for functional groups whose pKa is approximately 5.0 and lower.
What are the reagents used in TLC?Ferric Chloride / sulfuric acid Used as a charring reagent for polymer bound TLC plates (the newer hard layer plates) Spray with a solution of 2g FeCl3 in 83ml n-butanol and 15ml conc. sulfuric acid.
How do you make TLC solution?2.3E: Step-by-Step Procedures for Thin Layer Chromatography Dissolve the Samples. Prepare the TLC Chamber and Plate. Spot the TLC plate with sample. Place the TLC plate in the chamber to "elute" Remove the TLC plate from the chamber.
How do you prepare KMnO4 solution for TLC stain?KMnO4 Olefins and other readily oxidized groups. Dissolve 1.5 g KMnO4, 10 g signNowO3, and 1.25 mL 10% NaOH in 200 mL water. Cerium Sulfate General stain, particularly useful for alkaloids. Make an aqueous solution of 10% Cerium (IV) sulfate and 15% H2SO4.
How do you prepare PMA solution for TLC?Phosphomolybdic Acid (PMA): Dissolve 10 g of phosphomolybdic acid in 100 mL of absolute ethanol. Potassium Permanganate: Dissolve 1.5 g of KMnO4, 10 g of signNowO3, and 1.25 mL of 10% NaOH in 200 mL of water. Vanillin: Dissolve 15 g of vanillin in 250 mL of ethanol and add 2.5 mL of conc. sulfuric acid.