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Titration of Fluorochrome Conjugated Antibodies for Labeling Cell Surface Markers Form

Titration of Fluorochrome Conjugated Antibodies for Labeling Cell Surface Markers Form

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when you need to label an antibody with a dye Pascal enzyme or protein there are several choices in this how-to video will illustrate a site-specific antibody labeling technique that utilizes click chemistry this technique is simple and at the end of this video you will be able to select the tools that you need to expertly create a site specifically labeled antibody by targeting carbohydrate groups on the antibody the label of your choice is specifically conjugated to the FC region of the antibody the specific targeting maintains the integrity of the antigen binding site on the antibody these products are called cyclic antibody labeling kits click chemistry is a method used to assemble molecules using a Zeitz and alkynes the reaction is simple in three basic steps you'll have a conjugated antibody ready for use in the first step the enzyme beta-galactosidase exposes clerk nak residues by removing terminal galactose residues from the carbohydrate sites in the FC region in the se

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