MLG 8 10 Isolation and Identification of Listeria Monocytogenes from Red Meat, Poultry, Ready to Eat Siluriformes Fish and Egg P
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People also ask
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What are the methods for identification of Listeria monocytogenes?
Current methods for identification of L. monocytogenes rely on physiological and biochemical methods. These include Gram stain morphology, catalase, motility, beta hemolysis on blood agar and oblique illumination of colonies on blood free agar (6). -
How would you isolate and identify Listeria monocytogenes?
monocytogenes involved the use of culture methods based on selective enrichment and plating. Isolates were identified by colony morphology, sugar fermentation and haemolytic properties. Genotypic identification of L. monocytogenes was performed using PCR incorporating 16S rRNA followed by DNA sequence analysis. -
How do you isolate Listeria monocytogenes from food?
Isolation of the Listeria spp The selective media used for isolation of L. monocytogenes is Oxford agar. The media were ready as per the composition (Table 2). The culture (1ml) is taken from enrichment medium and streaked on the Oxford agar plates and incubated at 37°C for 48 hours. -
What are the identification methods of Listeria monocytogenes?
Current methods for identification of L. monocytogenes rely on physiological and biochemical methods. These include Gram stain morphology, catalase, motility, beta hemolysis on blood agar and oblique illumination of colonies on blood free agar (6). -
What characteristic features can be used to identify Listeria monocytogenes?
Listeria spp. are small gram-positive rod (0.5–4 μm in diameter and 0.5–2 μm in length), non-spore-forming, facultative anaerobic, catalase-positive, and oxidase-negative organisms. Listeria has tumbling motility at 20–25°C due to peritrichous flagella. -
How can Listeria be isolated in the lab?
For optimal isolation of Listeria species, direct plating onto blood agar is recommended. This may be direct from clinical specimens or from subcultures of blood culture medium. In addition, when foodborne cases are being investigated, culture from faeces is often useful. -
What is MLG in food safety?
The MLG contains methods for the sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the detection of antimicrobial residues. -
How do you isolate Listeria monocytogenes in food?
Isolation of the Listeria spp The selective media used for isolation of L. monocytogenes is Oxford agar. The media were ready as per the composition (Table 2). The culture (1ml) is taken from enrichment medium and streaked on the Oxford agar plates and incubated at 37°C for 48 hours. -
What are the methods of detection of Listeria monocytogenes?
Molecular detection methods developed for L. monocytogenes include PCR, quantitative PCR (qPCR), and multiplex qPCR (Long et al., 2008; Bickley et al., 2010; Chen et al., 2011; Witte et al., 2016). -
What are the traditional methods for isolation of Listeria monocytogenes?
Conventional methods for the detection of Listeria monocytogenes in foods and environmental samples relies on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers. -
How do you identify Listeria monocytogenes in a lab?
The diagnosis of L. monocytogenes requires a culture of the bacteria from the blood, cerebral spinal fluid, or placental fluid. Once in the lab, Listeria species grows on a special type of agar called Meuller-Hinton agar. Culture will reveal gram-positive rods with colonies that are beta-hemolytic. -
How do you isolate and identify Listeria monocytogenes?
monocytogenes involved the use of culture methods based on selective enrichment and plating. Isolates were identified by colony morphology, sugar fermentation and haemolytic properties. Genotypic identification of L. monocytogenes was performed using PCR incorporating 16S rRNA followed by DNA sequence analysis.
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