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Restriction Mapping  Form

Restriction Mapping Form

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As the society takes a step away from office work, the execution of paperwork more and more occurs online. The restriction mapping form isn’t an any different. Dealing with it using electronic means is different from doing this in the physical world.

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Video instructions and help with filling out and completing Restriction Mapping Form

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Instructions and help about Restriction Mapping

this is a quick tutorial in how to do restriction mapping and what i mean by that is when you have a plasmid that you've digested with some restriction enzymes how to interpret the bands and draw a map of the plasmid from those bands so here we have a hypothetical gel what you have is the wells at the top of the gel you've got five different lanes lane one is your dna ladder and we've marked um all of the fragments on the ladder 700 base pairs 500 base pairs etc lane 2 is the undigested plasmid and it's 700 base pairs lane 3 is the plasmid digested with eco r5 lane 4 is the plasmid digested with bam h1 and lane 5 is the plasmid digested with both eco r5 and bam h1 and so the question is how do you interpret this data and get to a map of the plasmid that has the locations or the relative locations of the eco r5 restriction sites and the bam h1 restriction sites all right i have redrawn the gel and labeled each of the lanes so let's see how we can actually map this plasmid we know our wh

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