Mono Q 5 50 Gl Manual Form
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People also ask
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What is the binding capacity of protein A beads?
Binding Capacity 1 ml of Protein A Magnetic Beads binds > 280 µg of Human IgG.
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How does anion exchange chromatography work?
Anion exchange chromatography is a form of ion exchange chromatography (IEX), which is used to separate molecules based on their net surface charge. Anion exchange chromatography, more specifically, uses a positively charged ion exchange resin with an affinity for molecules having net negative surface charges.
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How to clean a monoq column?
For column storage, wash with 5 column volumes of distilled water followed by 5 column volumes of 20% ethanol. Degas the ethanol/water mixture thoroughly and apply at a low flow rate to avoid over- pressuring the column.
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What is the binding capacity of anti flag resin?
The ANTI-FLAG® M2 Magnetic Bead resin is supplied as a 50% suspension in 50% glycerol with 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, and 0.02% (w/v) sodium azide. Binding capacity: 0.6 mg of FLAG® fusion protein per 1 mL of packed magnetic beads.
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What is the binding capacity of Monoq?
MonoBeads media are porous, with charged surfaces within the beads as well as on the outside. This gives MonoBeads a high binding capacity, particularly for Mono Q and Mono S, which can bind up to 50 mg globular protein/ml medium. Mono P columns can bind up to 10 mg globular protein/ml medium.
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What is the binding capacity of the streptavidin beads?
Binding Capacity The beads will bind greater than 1000 pmol of free biotin per mg and greater than 500 pmol of single-stranded 25 bp biotinylated oligionucleotide per mg.
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What is the binding capacity of Qiaquick?
Specifications FeaturesSpecifications Binding capacity 10 µg Technology Silica technology Sample type: applications ssDNA or dsDNA from PCR and other enzymatic reactions Fragments removed < 40mers5 more rows
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