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Below is a Restriction Map for the Plasmid Pgen101  Form

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Instructions and help about restriction mapping with excel

okay let's take a look at another example on how to build restriction site maps on plasma dna so this plasmid has been already digested and you can see the data is provided here we have different fragment sizes that resulted from different digestions so we have single digestions going on and double digestion so the very first thing that we want to do again we want to establish how big this plasmid is so we want to look at single digestion and you can see here is just one fragment and you can see we only have 3 000 base pairs and then here's another one from [Music] enzyme number two digestion again three thousand base pairs but if we have two fragments uh notice they do add up to three thousand and if you add all these fragments together you're going to see that they all add up to 3000 base pairs so it means this is how big our plasmid is 3 000 base pairs total so now the very first thing um after that or the next thing that you want to do after that is figure o

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