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Tdtomato Universal Genotyping Primer Form

Tdtomato Universal Genotyping Primer Form

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[Music] very good morning and welcome to explore bio if you are working on a model organism such as human mice arabidopsis drosophila you have access to their genomic information for primary designing but if you are working on a species with limited or no sequence information and you want to amplify or clone a gene what will you do is it possible to design primer for such genes for which the sequence is not available in today's video i will talk about degenerate primers and how to design them for pcr amplification or cloning of genes in species liking the sequence information if you want to amplify a gene or clone it you need to have its complementary primer pair which is easy in case of model organisms with gene sequences available in case of non-modal plants with limited sequence information you can take the sequence of target gene from closely related species which belong to the same genus or family this is done by performing blast homology search i have made a separate tutorial on

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