Amplitaq Gold 360 Protocol Form
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People also ask
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What does Amplitaq Gold DNA polymerase do?
This modified enzyme is provided in an inactive state and upon thermal activation the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.
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How do you activate the enzyme in amplitaq gold?
The activation is performed by heating the enzyme reaction mix for 1 to 10 minutes at 95° C. Upon activation, the modifier is permanently released, regenerating the active enzyme and initiating PCR amplification.
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What is Taq DNA polymerase used for?
Taq polymerase is specifically used in PCR to recruit new nucleotides to the single strands of DNA brought about by the denaturation step of the PCR reaction to complete DNA replication.
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What is the function of the Taq buffer in PCR?
Taq buffer is a crucial component in PCR, a technique that's used to produce multiple copies of a specific DNA sequence. The buffer contains various chemicals that help regulate the pH level and also provide essential elements for the enzyme to work correctly so that the DNA can be copied and amplified correctly.
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What is the purpose of the DNA polymerase?
The primary role of DNA polymerases is to accurately and efficiently replicate the genome in order to ensure the maintenance of the genetic information and its faithful transmission through generations.
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What is the error rate of AmpliTaq gold?
Keep in mind that the PCR error rate of AmpliTaq Gold is 1 error in every 10^5 bases.
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