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[Music] thank you the overall goal of this procedure is to construct multi-gene synthetic operons under the control of an inducible t7 RNA polymerase for expressing multiple proteins simultaneously this method can help researchers in synthetic biology and biochemistry enabling them to build multi-protein Pathways or study protein complexes that are dependent on co-expression of multiple proteins the main advantage of this technique is that it allows multiple genes to be expressed simultaneously in ashirika coli from a single plasma demonstrating the procedure from my lab will be Mohamed Hassan a PhD student and Angela Thompson a co-op student after obtaining and amplifying genes of Interest ing to the text protocol prepare digestion reactions for the first Gene of interest and the pmgx vector using a 40 microliter reaction containing 0.5 to 1.5 micrograms of DNA and the endel enzyme incubate the reactions at 37 degrees Celsius for one hour then add Echo R1 and allow the digest to proce

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