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Application Note Selecting Reagents for Multicolor Flow Cytometry Form
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People also ask
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What should you consider when choosing fluorophores for flow cytometry?
Selecting the Right Fluorophores for Flow Cytometry Experiments Understand your flow cytometer. ... Consider target abundance. ... Research fluorophore properties. ... Increase panel size with tandem dyes. ... Think about using calibration and compensation beads. ... Never underestimate the importance of compensation controls. -
What are the application of flow cytometry notes?
Flow cytometry is a powerful tool that has applications in multiple disciplines such as immunology, virology, molecular biology, cancer biology and infectious disease monitoring. For example, it is very effective for the study of the immune system and its response to infectious diseases and cancer. -
What are the reagents used in flow cytometry?
/FITC, APC, cyanine, PE, and TRITC are some of the most well known, although new brands continually become available. These fluorophores can be found conjugated to secondary antibodies or other molecules such as Protein A, Protein G, or streptavidin. -
What is the principle of multicolor flow cytometry?
In multicolor flow cytometry, fluorescent markers are used to characterize and define different cell types of interest in heterogeneous cell populations, assess the purity of isolated subpopulations, and analyze cell size and shape.
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