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Application Note Selecting Reagents for Multicolor Flow Cytometry Form
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People also ask
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What are the clinical applications of flow cytometry?
Flow cytometry is most commonly indicated for both benign and malignant hematologic processes. It can aid in several clinical areas, including diagnosis, treatment plans, and monitoring residual or relapsed disease ((Craig & Foon, 2008; (Wood et al., 2007).
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What are the reagents used in flow cytometry?
Agilent offers an extensive selection of conjugated antibodies intended for use in immunophenotyping by flow cytometry. Each antibody is conjugated with one of various commonly used fluorochromes such as FITC or APC and target specific cell lineage markers.
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What should you consider when choosing fluorophores for flow cytometry?
The fluorophore performance and its utility in a multicolor panel depend on the following factors: Excitation and emission profiles. Relative brightness. Quantum yield. Emission overlaps. Stability of antibody-fluorophore conjugate. Reproducibility of antibody conjugation.
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What are the applications of imaging flow cytometry?
The technology has several applications, including assessment of cell cycle stages based on nuclear DNA content and morphology; detection and characterization of small particles like exosomes or microvesicles that may be present with the cells; morphological and phenotypic profiling of cells, including size, shape, and ...
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What is the main use of flow cytometry?
Flow cytometry is a laser-based lab test that can detect chemical and physical differences of cells or particles. Healthcare providers commonly use it to evaluate bone marrow, peripheral blood and other fluids in your body. Flow cytometry can help diagnose several conditions, including infectious diseases and cancer.
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What are the major applications of flow cytometry?
The most common application performed on the cytometer is immunophenotyping. This technique identifies and quantifies populations of cells in a heterogeneous sample - usually blood, bone marrow or lymph. These cell subsets are measured by labeling population-specific proteins with a fluorescent tag on the cell surface.
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What is the principle of multicolor flow cytometry?
The multicolor element of flow cytometry relies on multicolor panels that require the use of tandem dyes. Tandem dyes are made up of a donor fluorophore that generates excitation characteristics, and another acceptor fluorophore that creates the emission characteristics.
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What is the most common application of flow cytometry?
The most common application performed on the cytometer is immunophenotyping. This technique identifies and quantifies populations of cells in a heterogeneous sample - usually blood, bone marrow or lymph.
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