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Exceptions to CLA Major or Minor Form, DOC Development of the Fruit Fly, Drosophila Melanogaster D Umn

Exceptions to CLA Major or Minor Form, DOC Development of the Fruit Fly, Drosophila Melanogaster D Umn

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[Music] the overall goal of this procedure is to rear fruit flies that are free of associated microorganisms this method can help any researcher in the drosophila field control for manipulate or study the microbiota including its relationship to various host traits the main advantage of this technique is that it permits researchers to rear fruit flies in the complete absence of external microorganisms generally individuals new to this method will struggle because the sterilization and rinsing steps must be conducted without introducing any contaminating microbes to begin the experiment streak previously prepared cultures on modified mrs or mmrs agriplates incubate the plates overnight at 30 degrees celsius incubate anaerobic cultures in an airtight container and flood it with carbon dioxide before sealing the next day pick a single colony from the mmrs plate into a test tube containing mmrs broth grow lactobacillus species under static conditions and grow acetobacter species with shaki

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