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People also ask
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Which of the following is a step in rho-dependent termination?
In a nut-shell, Rho-dependent termination has three basic steps, a) Rho loading b) Rho translocation and c) Rho unwinding of DNA-RNA duplex to release RNA. These basic steps are often influenced by several known (like NusG) and unknown cofactors.
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What are the steps of Rho-dependent termination?
In a nut-shell, Rho-dependent termination has three basic steps, a) Rho loading b) Rho translocation and c) Rho unwinding of DNA-RNA duplex to release RNA. These basic steps are often influenced by several known (like NusG) and unknown cofactors.
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What is the rho-independent termination sequence?
Rho-independent (also known as intrinsic) terminators are sequence motifs found in many prokaryotes that cause the transcription of DNA to RNA to stop. These termination signals typically consist of a short, often GC-rich hairpin followed by a sequence enriched in thymine residues [1].
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What is the rut site sequence?
Rho utilisation site, also known by the acronym rut, is a sequence of RNA in bacteria upstream of the terminator region which serves as a binding site for the protein known as rho factor. This sequence is necessary in rho-dependent termination of DNA transcription in bacteria.
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What is dependent termination?
Rho-dependent termination occurs by binding of Rho to ribosome-free mRNA, C-rich sites being good candidates for binding. Rho's ATPase is activated by Rho-mRNA binding, and provides the energy for Rho translocation along the mRNA; translocation requires sliding of the message into the central hole of the hexamer.
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What is the process of rho-dependent termination?
Rho-dependent termination occurs by binding of Rho to ribosome-free mRNA, C-rich sites being good candidates for binding. Rho's ATPase is activated by Rho-mRNA binding, and provides the energy for Rho translocation along the mRNA; translocation requires sliding of the message into the central hole of the hexamer.
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What is the difference between intrinsic and Rho-dependent termination?
Prokaryotes use intrinsic termination, also known as rho-independent termination, to indicate the termination of transcription and release the newly created RNA molecule. In Rho-dependent termination, a Rho protein is responsible for transcriptional termination.
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What are the two types of transcription termination?
Transcription Cycle: Termination There are two types of termination: ρ-dependent termination and ρ-independent intrinsic termination (Roberts, 2019). The ρ-dependent termination requires a hexameric ATP-dependent helicase protein ρ, which loads onto nascent RNA strands at rut (rho utilization) sites (Fig.
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