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Application of Oligonucleotide Microarray for the Detection and Genotyping of Cry Genes in Bacillus Thuringiensis Form
Genomic DNA were fluorescent-labeled using a random primer. The
corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA
after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified
the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid
for the detection and genotyping of genes. This type of assay is also a potentially valuable...
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