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Application of Oligonucleotide Microarray for the Detection and Genotyping of Cry Genes in Bacillus Thuringiensis Form

Application of Oligonucleotide Microarray for the Detection and Genotyping of Cry Genes in Bacillus Thuringiensis Form

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Genomic DNA were fluorescent-labeled using a random primer. The corresponding oligonucleotide probes were designed for the different cry genes that can hybridize Bt genomic DNA after cluster analysis and were printed on glass slides. This microarray has unambiguously detected and identified the cry genes in 10 isolates and reference Bt. Our data demonstrates that the microarray assay is simple and rapid for the detection and genotyping of genes. This type of assay is also a potentially valuable...
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Hello back then welcome back to another video from my YouTube channel that is within Sharma biology tutorials, and today we are learning a very important topic from chapter biotechnology of 12 class that are try genes so before understanding the concept behind cry genes let us now go through several statements which are very important, so the statements are specific Beauty toxins incorporated into several crops like cotton specific Beauty toxins specific because these are insect pest specific if you want to kill a particular species of insect pest then you have to choose specific BT toxin different kind of toxins are there to kill specific insect pests so specific be detoxes BT is arisen from bacillus thuringiensis to be from bacillus and tea from thuringiensis so specific BT toxins are several proteins or several you know chemicals which are extracted from this bacillus thuringiensis which are specific in nature, so those specific beauty boxes incorporate that means enters into severa

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