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Illumina Laboratory Form

Illumina Laboratory Form

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Instructions and help about illumina clinical laboratory

The Illumina sequencing workflow is composed of 4 basic steps– sample prep, cluster generation, sequencing and data analysis. There are a number of different ways to prepare samples. All preparation methods add adaptors to the ends of the DNA fragments. Through reduced cycle amplification, additional motifs are introduced, such as the sequencing binding site, indices and regions complementary to the flow cell oligos. Clustering is a process where each fragment molecule is isothermally amplified. The flow cell is a glass slide with lanes. Each lane is a channel coated with a lawn, composed of two types of oligos. Hybridization is enabled by the first of the two types of oligos on the surface. This oligo is complimentary to the adapter region on one of the fragment strands. A polymerase creates a complement of the hybridized fragment. The double stranded molecule is denatured and the original template is washed away. The strands are clonally amplified through bridge amplification. In t

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