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Mtcc Culture Data Sheet Form

Mtcc Culture Data Sheet Form

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These HEK293 cells were cultured in a tissue   culture flask and maintained between 10  and 90% confluency in a humidified 37°C,   10% CO₂ incubator. The researcher confirms  the correct density of cells using microscopy. Cell culture medium is removed from the cells,  then the cells are washed one time with PBS.   For a T75 flask, 2 mL of TrypLE trypsin  solution is added to detach the cells   from the plate. The cells are incubated  with the trypsin solution for 2 minutes. After incubation, the researcher verifies  under a microscope that the cells are   detached and that all clumps are dispersed.  Trypsinization is stopped by adding 10 mL   of assay medium per T75 flask. Cells in assay  medium are dispersed by pipetting up and down. The cell suspension is transferred  to a conical tube. A hemocytometer   is used to quantify the number  of cells in solution. Cells are   counted in order to determine their  concentration for subsequent assays. The cells a

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