
PD 10 Desalting Columns GE Healthcare Life Sciences Form


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People also ask
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What is the difference between buffer exchange and desalting?
Desalting occurs when buffer salts and other small molecules are removed from a sample in exchange for water (with the resin being pre-equilibrated in water). Buffer exchange occurs when the buffer salts in a sample are exchanged for those in another buffer.
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What is the purpose of the buffer exchange?
Buffer exchange is a simple yet important technique in most macromolecule purification workflows. It can be used to remove unwanted substances, adjust sample conditions, or introduce components to a sample.
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What is a PD-10 column?
PD-10 Columns are economical, plastic columns for gravity flow purification. Economical and versatile columns for gravity flow applications.
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What is the desalting column method?
Desalting columns use the principle of size exclusion chromatography, also called gel filtration chromatography. In addition to desalting, the other main use for size exclusion chromatography is size fractionation. The matrix beads in a size exclusion column contain pores.
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What was the role of buffer exchange column chromatography using a PD-10 column?
PD-10 Desalting columns are designed for convenient desalting and buffer exchange of 1.0 to 2.5 mL volume of protein sample. The columns are prepacked with Sephadex G-25 Medium, an SEC medium that allows effective removal of low molecular weight substances from proteins with Mr > 5000.
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What is the use of PD-10 column?
Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.
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What is the volume of a pd10 sample?
The PD-10 Desalting column is intended for sample volumes up to 2.5 mL. For sample volumes less than 2.5 mL, allow the sample to enter the packed bed completely and then add equilibration buffer (stacker volume) so that the total volume of sample and buffer added equals 2.5 mL.
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Can PD-10 columns be reused?
PD10 can be used many times even without cleaning. Generally I monitor the flow rate and when the column starts to perform too slowly then I will replace it.
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