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Arrest in Transfection Reagent Form

Arrest in Transfection Reagent Form

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there are a number of different methods out there to help us do transaction early on a lot of people use calcium phosphate precipitate to transfect cells you had to get the pH just right in the transactions weren't that great then Along Came cationic lipid reagents and everything got much easier now you just mix the DNA or si RNA with the lipid and add it to your cells very easy you mix the two and the negatively charged nucleic acid molecules bind the cationic lipid and form a complex the combined cells and be taken or through the endocytic pathway once in the endosome the nucleic acid need to escape into the cytoplasm before being degraded the center soma escape occurs by many different mechanisms including membrane fusion and disruption by interactions and lipid exchange between the transfection reagents and the endosomal membrane once the endosome is disrupted the nucleic acid is free in the cytoplasm and available for nuclear shuttling in the case of DNA or binding the RIS see com

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