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Pet16b Form

Pet16b Form

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in this video I'm going to show you how to read a plasmid vector map to determine the fragment lengths you would get after a restriction enzyme digest this is a map of a plasmid vector in the middle it tells you its name it says it's P gem which is the name of the vector and after the - that's the name of the gene that's been inserted here it's the thick gray line is our inserted gene H n R NP a2 and then in the other number the 4220 base-pairs tells you how big is this plasmid and there's a lot of stuff going around on the outside that what those things tell us are the restriction enzyme sites how many there are and then some other information like where the primers are that made the gene that we were inserted here but what we're concerned with for this video is how could we tell if we were going to do a restriction digest well how big would our fragments be when we run them out on a gel so we know the whole thing is four thousand two hundred and twenty base pairs but where is the beg

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