H21 A4 Collection, Transport, and Processing of Blood Specimens for Testing Plasma Based Coagulation Assays; Approved GuidelineF Form
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People also ask
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What are some specimen collection requirements for coagulation studies?
The specimen of choice for coagulation testing is plasma. Venous blood is drawn into a 3.2% buffered sodium citrate tube (blue top tube), yielding a whole blood sample with a 9:1 blood to anticoagulant ratio. Inadequate filling of the collection tube will decrease this ratio, and may affect test results. -
What is normal range for platelet poor plasma?
Ideally, PPP should have a platelet count of less than 10 x 10E9 per liter (<10,000 /ul). Specimens requiring Platelet Poor Plasma (PPP) must be collected and handled in the following manner: Specimen Collection: Obtain venous blood by drawing a clearing tube prior to obtaining the specimen. -
What should blood collected for coagulation testing be collected in?
Sample Collection for Coagulation Testing. 1. Collection Tube. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate. -
What is the CLSI guideline h21 a4?
The guideline provides procedures for the collection, transport, and processing of blood specimens for coagulation testing. Tests of the coagulation system are very sensitive to storage (time and temperature), concentration of anticoagulant, and surface of containers; attention to these parameters is important. -
What precautions should be considered in collecting blood for coagulation studies from an indwelling catheter?
When specimens are collected from indwelling catheters, in order to prevent possible contamination or specimen dilution, the line should be flushed with 5 mL of saline. The first 5 mL of blood or 6-times the line volume (dead space volume) of the catheter must be discarded before the coagulation tube is filled. -
What is the CLSI guideline h21 a4?
The guideline provides procedures for the collection, transport, and processing of blood specimens for coagulation testing. Tests of the coagulation system are very sensitive to storage (time and temperature), concentration of anticoagulant, and surface of containers; attention to these parameters is important. -
What tests require platelet poor plasma?
Clinical and Laboratory Standard Institute guidelines require that routine coagulation tests are performed with platelet-poor plasma (< 10,000 platelets/microL) and prepared from whole citrated blood centrifuged at low speed for 10-30 minutes. -
What are the CLSI guidelines for platelet poor plasma?
The international guidelines for pre-analytical handling of coagulation tests, from the Clinical and Laboratory Standards Institute (CLSI), recommend centrifugation of the samples to produce platelet-poor plasma (PPP), defined as a plasma platelet count lower than 10 × 109/L [7]. -
What is platelet poor plasma mean?
What is Platelet Poor Plasma (PPP)? PPP is a biologic treatment that is derived from your own blood. It is similar to platelet rich plasma (PRP) except that it has a lower concentration of platelet cells and growth factors. Medical literature has shown that some injuries such as muscle tears may heal better with PPP. -
Which of the following blood collection tubes is appropriate for coagulation testing?
The "citrate tube" is for collecting blood for performing coagulation studies. It contains 3.2% sodium citrate as its anticoagulant. -
How do you prepare specimens for coagulation tests?
If multiple tests are being drawn, draw coagulation studies before additive-containing tubes such as the EDTA, , or clot activator (SST) tubes. If only the coagulation specimen is being drawn, draw 1-2 mL into another blue top (3.2% sodium citrate) Vacutainer®, discard, and then collect the specimen.
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