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UNIVERSITY of CALIFORNIA SAN DIEGO, MOUSE HISTOLOGY Form

UNIVERSITY of CALIFORNIA SAN DIEGO, MOUSE HISTOLOGY Form

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[Music] the overall goal of the following video is to present an image registration approach for three-dimensional histology volume reconstruction to study changes in mammary cellular macrostructure to do this mammary glands are excised from mice three days after the onset of lactation in wild type and insulin-like growth factor binding protein 7 igf-bp-7 null mice the glands are then processed and embedded in paraffin blocks for sectioning next optical block face images of the tissue blocks are captured and the tissue block is sectioned images of the sections are captured and using custom software each section is aligned with its corresponding block face image to reconstruct histology volume of the mammary gland the resulting data highlight differences in size in mutant and wild-type glands the main advantage of this technique over existing methods like three-dimensional confocal two-photon microscopy is that it provides information over a much greater spatial extent this method can a

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