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Triple Helix Formation with the Promoter of Human 1I Procollagen Gene by an Antiparallel Triplex Forming Oligodeoxyribonucleotid
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People also ask
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What is the triple strand of DNA?
The triple-stranded DNA (H-DNA) was predicted to be composed of one polypurine and two polypyrimidine strands. It was thought to occur in only one in vivo biological process: as an intermediate product during the action of the E. coli recombination enzyme RecA. -
What is the structure of triplex DNA?
A DNA triplex is formed when pyrimidine or purine bases occupy the major groove of the DNA double Helix forming Hoogsteen pairs with purines of the Watson-Crick basepairs. Intermolecular triplexes are formed between triplex forming oligonucleotides (TFO) and target sequences on duplex DNA. -
What is the TFO in triplex DNA?
A DNA triplex is formed when pyrimidine or purine bases occupy the major groove of the DNA double Helix forming Hoogsteen pairs with purines of the Watson-Crick basepairs. Intermolecular triplexes are formed between triplex forming oligonucleotides (TFO) and target sequences on duplex DNA. Triplex DNA structures - PubMed nih.gov https://pubmed.ncbi.nlm.nih.gov › ... nih.gov https://pubmed.ncbi.nlm.nih.gov › ... -
What is TFO in genetics?
Triplex-forming oligonucleotides (TFOs) can bind to the major groove of double-stranded DNA with high specificity and affinity and inhibit gene expression. Triplex-forming oligonucleotides have gained prominence because of their potential applications in antigene therapy. -
What is the triple helical structure of DNA?
A triple helix is formed after the binding of a third strand to the major groove of a duplex DNA through Hoogsteen base pairing. Pyrimidine-rich and purine-rich sequences can form stable triplex structures as a consequence of the formation of A–T–A and C+–G–C triplets (Fig. -
What is the function of the triplex DNA?
Naturally occurring intramolecular triplexes play important roles in regulating DNA metabolism and gene function, and are inherently mutagenic and recombinogenic. DNA Triple Helices: biological consequences and therapeutic ... nih.gov https://.ncbi.nlm.nih.gov › articles › PMC2586808 nih.gov https://.ncbi.nlm.nih.gov › articles › PMC2586808
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