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welcome to the Intriguing world of molecular biology today we're delving deep into the polymerase Chain Reaction or PC ra groundbreaking technique that has transformed microbiological research join us on a detailed journey to understand how PCR works and its astounding applications in microbiology PCR is not just a lab technique it's a doorway to understanding life at the molecular level developed by Carrie Mullis in 1983 this method allows us to amplify minute quantities of DNA making it visible for study and Analysis let's explore the science behind PCR it involves multiple steps denaturation annealing and extension each step occurs at specific temperatures and plays a vital role in DNA amplification denaturation DNA unwinds at high temperatures separating into two strands a nealing primers bind to specific sequences on these single strands extension DNA polymerase extends the primers creating new DNA strands essential components of PCR include the DNA templat

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