Multiple Forms of Phosphoinositide Specific Phospholipase C of Ncbi Nlm Nih

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Hello, in this video we will see phospholipase C : IP3 / DAG pathway of intracellular signaling. Let's get started. This pathway is seen downstream of Gq protein. Initial events in this pathway are similar to other G proteins that we have seen in the video on G protein-coupled receptors. Under resting state, the trimer of α, β, and γ subunit is bound to the receptor and α subunit is bound to GDP. The binding of a ligand with the receptor causes conformational changes that cause release of GDP and binding of GTP. This leads to disassembly of subunits and formation of free α subunit and β-γ complex. The α subunit travels along the membrane and goes to phospholipase C. Phospholipase C is a membrane-bound enzyme. α subunit activates this enzyme. The active enzyme then interacts with phosphatidylinositol 4,5 bis-phosphate or PIP2 in short. Now, this is PIP2 is one of the phospholipids found in the cell membrane. It's mainly distributed in the inner leaflet of the bilayer. Phospholi