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Detection of Specific Lytic and Latent Transcripts Can Help to Predict Actabp  Form

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[Music] the overall goal of this procedure is to allow a detailed analysis of the phosphorylation dependent activation of the irf3 transcription factor so this method can help answer key questions regarding the interferon-mediated innate immune response such as our pathogen activates or evades the rf3dependent interferon beta expression the main advantage of this technique is that it constitutes an affordable and sensitive approach to distinguish the different rf-3 activated form that may arise following different stimulations though this method provides insight into virus infection of human cells it can also be applied to mouse or human tissues subjected to other pathogens or pathogen-associated molecular patterns demonstrating the procedure will be alexa ravitay and melisa mariani two cell signaling experts from my laboratory the a549 cells used in this procedure are maintained in culture in a 15 centimeter plate at 37 degrees celsius and 5 carbon dioxide and complete f12k ham medium

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