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People also ask
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What is the principle of oligonucleotide ligation assay?
The OLA is based on the covalent joining of two adjacent oligonucleotides by a DNA ligase when they are hybridized to a cDNA target.
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What is the principle of proximity ligation assay?
Methods of Analysis The Proximity Ligation Assay (PLA) depends on a simultaneous detection of targets by probes attached to secondary antibodies; when the ends of these probes are in close proximity to hybridize together a ligation reaction occur.
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What is an oligonucleotide ligation assay and how does it work?
The oligonucleotide ligation assay (OLA) is an inexpensive SNP assay that detects ligation between a biotinylated "allele-specific detector" and a 3′ fluorescein-labeled "reporter" oligonucleotide. No ligation occurs unless the 3′ detector nucleotide is complementary to the SNP nucleotide.
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What is the principle of antisense oligonucleotides?
The antisense concept is based on an understanding of nucleic acid structure and function, and depends on Watson–Crick hybridization mechanisms2. Antisense oligonucleotides are designed to inhibit the translation of RNA specifically by binding with high affinity and selectivity to their RNA targets (Fig.
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What is the basic principle of ligation?
Ligation involves joining up the ends of a DNA with other ends, however, each DNA fragment has two ends, and if the ends are compatible, a DNA molecule can circularize by joining its own ends.
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What is oligonucleotide signature sequence?
Oligonucleotide signatures (signatures) are short sequence strings (λ-mers) of fixed length (signature length λ), normally 18 to 100 bp, that match exactly and exclusively one or more targeted sequence(s) (targets) in a given genetic data set, usually from the same region of the genomes of targeted taxa.
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