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Tetrahedron Letters Vol 21, Pp LZ7 140 Jay Ponder Lab Dasher Wustl  Form

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let's take just a moment to look at the software interface for all ago seven all ago seven is one of these tools that we've mentioned for primer design now we're looking at a demo that's made available so this is this is not the full-featured function that would let us load arbitrary sequences we're just working through the demo provided with the the code itself so we see that a sequence of 1868 nucleotides has been imported into the software this is a reverse transcription of the mature messenger RNA for human as with specifically the eukaryotic translation initiation factor for e now as we look at this whole sequence we can see that we know the reading frame where our sequence is the translated sequence comes from is reading frame number one and that are currently our current location is sitting at a current I'll ago length the primer length that we're looking at is 21 nucleotides and you can move that around arbitrarily within

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