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Caveolins in Vascular Smooth Muscle Form Organizing Function Cardiovascres Oxfordjournals

Caveolins in Vascular Smooth Muscle Form Organizing Function Cardiovascres Oxfordjournals

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[Music] thank you [Music] the overall goal of this experiment is to characterize plant cell wall polysaccharides this method can help us answer key questions in the plant cell wall Biology field including glucomannon structure and quantity the main advantage of this technique is that it doesn't require specialized training or expensive equipment generally individuals new to this method will struggle with interpretation of the gels so inclusion and understanding of all the controls described is critical the alcohol insoluble residue or air was previously prepared from harvested plant material as described in the text protocol weigh six milligrams of air into a 15 milliliter centrifuge tube and add water to a final concentration of two milligrams per milliliter Vortex to achieve an even suspension aliquot 500 micrograms into microfuge tubes dry aliquots using a vacuum centrifuge at 30 degrees Celsius overnight pre-treat the air including an aliquot for a no enzyme air control wit

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