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Lab Specific Training and Checklist for New Lab Workers Lisef Stonybrook  Form

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hello and welcome to part one of our lab skills series working with cryo-preserved cells brought to you by california north state university college of pharmacy before you retrieve your cells make sure the sash of the biosafety cabinet is at the proper level to allow for ideal airflow sterilize the hood with 70 ethanol and have all required supplies at hand to minimize airflow disruption try to work in the middle of the hood space take care when removing your cryotube from the liquid nitrogen always use protective gloves and a face shield or goggles if available slowly pull the rack from the liquid nitrogen the tank is kept quite full and pulling the rack out quickly will cause the liquid nitrogen to splash and spill from the boxes pulling the rack out slowly allows for excess liquid nitrogen to drain back into the tank the cryotube should be put on ice immediately to prevent melting which will impact viability swirl the tube in a 37 degree water bath to melt the freezing media approxi

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